New Client Information Form
NAPCore FTP Server: napcore.res.chop.edu is no longer available. If you don't get your sequencing data, please call NAPCore at 215-590-3897 or email to email@example.com.
premixed format only!
The premixed format:
You submit DNA template and primer premixed in a tube. (If the primer is a standard primer available from the Napcore, you don't have to add the primer)
For one sequencing rxn, mix the DNA and primer according to the table below:
|DNA template||DNA and primer|
|PCR product||mix 10 - 100 ng DNA and 2 picomole primer (1 ul of 2 uM ) in a total of 9 ul.
10 ng per 100 bp.
i.e. for 400 bp product, 40 ng DNA is needed for sequencing.
100 ng is needed for fragments > 1 kb.
|plasmids/phagemids/ M13phage etc.||mix 200 ng DNA and 2 picomole primer (1ul of 2 uM) in a total of 9 ul|
|large insert clones (size: 50 - 300 kb)
such as cosmid, BACs, PACs etc.
|The premixed format cannot be used to submit large insert clones. Please use the traditional format to submit such template.|
You fill out the premixed DNA sequencing request form.
Upon receiving your samples, we will use your premixed sample directly in sequencing rxn.
It is our policy to rerun all reactions that don't work, this only applies to the same submission, it would be wise to provide twice as much DNA and primer as needed in case of rerun (18 ul instead of 9 ul).