About Us

The Children’s Hospital of Philadelphia Research Institute’s Real-Time PCR Core Facility is located in room 1207 Abramson Research Center. The facility provides real-time PCR chemistries consultation, experimental design consultation, assistance in data analysis and troubleshooting. For questions regarding the operation of the facility and other technical questions please contact realtime@email.chop.edu or 215-590-4422.

Currently the facility operates an Applied Biosystems QuantStudio 12K flex with Open Array capabilities, 4 SDS-7900HT, SDS-7500Fast and SDS-7500. All systems can be used for Absolute or Relative Quantitative analysis for numerous sample types, SNP’s and allelic discrimination assays, Plus/Minus assays and dissociation curve melting point analysis. Supported platforms include 96-well, 384-well, Taqman LDA cards and OpenArray. The satellite facility located in 3312 Colket Translational Research Building operates an additional 2 SDS-7900HT instruments supporting 384-well plates.

The facility does not setup reactions for Investigators, but support is available upon request. Machine operations are self-served for experienced users from individual labs. Trainings sessions are highly recommended for first time users. Please contact realtime@email.chop.edu to setup an appointment.